Compare the starter assays before describing your own. Every example below is computed from the same frozen fixtures the test suite verifies — no numbers are invented.
Quantify protein concentrations from a BCA standard curve on a 96-well plate — replicate means, R², and out-of-range flags included.
C3/D3 · 0.2476 A562 → 0.2368 mg/mL
Opens with built-in sample data.
Quantify protein concentrations from a Bradford (Coomassie) standard curve at A595 — replicate means, R², and out-of-range flags included.
Unknown 02 · 0.1425 A595 → 0.212 mg/mL
Opens with built-in sample data.
Fit a 4-parameter logistic standard curve to OD450 readings and back-solve sample concentrations in ng/mL.
sample02 · 0.6022 OD450 → 5.81 ng/mL
Opens with built-in sample data.
Fit a 4PL dose-response curve over a µM dose ladder and read off IC50 with a show-the-math line for every sample.
E4/F4 · 1.321 response → 0.04918 µM
Opens with built-in sample data.
Relative quantification by the ΔΔCt (Livak) method — technical-replicate means, ΔCt against a reference gene, and fold change against a calibrator sample, with undetermined wells and replicate spread flagged.
Treated-A IL6 · 4× · ΔΔCt -2 · replicate range 3.36–4.76
Opens with built-in sample data.
Initial rates from kinetic reads, nonlinear Vmax/Km fit with standard errors, Lineweaver–Burk reference plot.
Measured · 0.1 mM → 0.02666 AU/min
Opens with built-in sample data.
Blank correction, log-phase detection, growth rate, doubling time, lag time, per-well summary table.
A1 · 0.9091 1/h · doubling 0.762 h · lag 1.8 h
Opens with built-in sample data.
Read percent viability for every treatment group against the untreated control — background subtraction, replicate CV, and a dose + %viability hand-off for the IC50 tool included.
dose-01 · 99.08% · corrected mean 0.9425 · CV 0.221%
Opens with built-in sample data.
Fit any assay’s standards with linear, quadratic, or 4PL chosen by AIC, back-calculate unknown concentrations, and flag out-of-range results.
U1 · 0.5634 signal → 0.8 concentration
Opens with built-in sample data.
Background-subtract ImageJ/Fiji band intensities, normalize to a loading control by lane label, and report fold change vs control with replicate stats.
C-1 · 1.333× · Control
Opens with built-in sample data.